MICROPROPAGATION OF PHALAENOPSIS AND DORITAENOPSIS BY CULTURING SHOOT TIPS OF FLOWER STALK BUDS

被引:8
作者
TOKUHARA, K [1 ]
MII, M [1 ]
机构
[1] CHIBA UNIV, FAC HORT, MATSUDO, CHIBA 271, JAPAN
关键词
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Green Protocorm-like Bodies (PLB) with high multiplication capacity were induced from shoot tips of flower stalk buds having 1 or 2 leaf primordia using New Dogashima Medium (NDM) containing 0.1 mg l-1 alpha-naphthaleneacetic acid (NAA) and 1 mg l-1 6-benzylaminopurine (BAP). These PLB were subcultured on the same medium. More than 10,000 PLBs were obtained from a few buds on a single flower stalk within one year. After transfer onto NDM containing no plant growth regulator (PGR), the PLB developed into plantlets. The micropropagation method formulated in this study was applicable to 12 different genotypes. These results suggest that the methodology could be used on a commercial scale for vegetative propagation of Phalaenopsis and Doritaenopsis.
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页码:7 / 11
页数:5
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