M13 BACTERIOPHAGE DNA AS A PROBE FOR DNA FINGERPRINTING IN GRAM-POSITIVE MICROORGANISMS

被引：7

作者：

MITEVA, V ^{[1
]}

ABADJIEVA, A ^{[1
]}

IVANOV, P ^{[1
]}

GRIGOROVA, R ^{[1
]}

机构：

[1] ACAD SCI USSR,INST MOLEC BIOL,MOSCOW 123182,USSR

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1990年 / 13卷 / 04期

关键词：

DNA fingerprinting; Gram-positives; M13; probe;

D O I：

10.1016/S0723-2020(11)80232-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hypervariable nucleotide sequences were found in the genomes of some Gram-positive microorganisms, Bacillus thuringiensis, Bacillus subtilis, Bacillus stearothermophilus, Bacillus sphaericus, Lactobacillus helveticus, Streptococcus pyogenes, Streptomyces albogriseolus, and the Gram-negative Rhizobium meliloti after hybridization with 32P labeled M13 DNA. This is a further proof of the wide-spread distribution of these sequences. The DNA fingerprinting method allows differentiation between serotypes of the insectopathogenic bacteria studied. This approach may become an efficient tool in bacterial identification. © 1990, Gustav Fischer Verlag, Stuttgart · New York. All rights reserved.

引用

页码：350 / 353

页数：4

共 11 条

[1] RESTRICTION ANALYSIS OF THE STREPTOMYCES-GLAUCESCENS GENOME BY AGAROSE-GEL ELECTROPHORESIS [J].