FUNCTIONAL EXPRESSION OF THE RAT HEART-I NA+ CHANNEL ISOFORM - DEMONSTRATION OF PROPERTIES CHARACTERISTIC OF NATIVE CARDIAC NA+ CHANNELS

被引：60

作者：

CRIBBS, LL ^{[1
]}

SATIN, J ^{[1
]}

FOZZARD, HA ^{[1
]}

ROGART, RB ^{[1
]}

机构：

[1] UNIV CHICAGO,DEPT MED,CARDIOL SECT,5841 S MARYLAND AVE,CHICAGO,IL 60637

来源：

FEBS LETTERS | 1990年 / 275卷 / 1-2期

关键词：

cDNA expression; Na[!sup]+[!/sup] channel; Na[!sup]+[!/sup] current; Saxitoxin; Tetrodotoxin; Xenopus oocyte;

D O I：

10.1016/0014-5793(90)81470-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe the expression of functional Na+ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na+ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na+ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle. © 1990.

引用

页码：195 / 200

页数：6

共 24 条

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