TRANSFER OF A FOREIGN GENE INTO THE BRAIN USING ADENOVIRUS VECTORS

被引：480

作者：

AKLI, S

CAILLAUD, C

VIGNE, E

STRATFORDPERRICAUDET, LD

POENARU, L

PERRICAUDET, M

KAHN, A

PESCHANSKI, MR

机构：

[1] FAC MED CRETEIL,INSERM,CJF 91-02,8 RUE GEN SARRAIL,F-94010 CRETEIL,FRANCE

[2] INST COCHIN GENET MOLEC,INSERM,U129,F-75014 PARIS,FRANCE

[3] INST GUSTAVE ROUSSY,CNRS,URA 1301,F-94805 VILLEJUIF,FRANCE

来源：

NATURE GENETICS | 1993年 / 3卷 / 03期

关键词：

D O I：

10.1038/ng0393-224

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The ability of a replication-deficient adenovirus vector to transfer a foreign gene into neural cells of adult rats in vivo has been analysed. A large number of neural cells (including neurons, astrocytes and ependymal cells) expressed an E coli lacZ transgene for at least 45 days after inoculation of various brain areas. Injecting up to 3 x 10(5) pfu in 10 mul did not result in any detectable cytopathic effects - these were only observed for very high titres of infection (>10(7) pfu 10 mul-1). Adenovirus vectors therefore appear to be a promising means for in vivo transfer of therapeutic genes into the central nervous system.

引用

页码：224 / 228

页数：5

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