EVIDENCE FOR STIMULATION OF THE INOSITOL TRIPHOSPHATE-CA2+ SIGNALING SYSTEM IN RAT ENTEROCYTES BY HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI

被引：14

作者：

CHAUDHURI, AG ^{[1
]}

GANGULY, U ^{[1
]}

机构：

[1] NATL INST CHOLERA & ENTER DIS,CALCUTTA 700010,W BENGAL,INDIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1267卷 / 2-3期

关键词：

HEAT STABLE ENTEROTOXIN; INOSITOL TRIPHOSPHATE; CALCIUM ION; INTRACELLULAR CONCENTRATION; EPITHELIAL CELL;

D O I：

10.1016/0167-4889(95)00055-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Escherichia coli heat stable enterotoxin (STa)-treated rat enterocytes, the rise of inositol triphosphate (IP3) preceded the rise of [Ca2+](i). Chelation of extracellular Ca2+ with EGTA and suspension of cells in Ca2+ free buffer both demonstrated the enterotoxin-induced initial rise of [Ca2+](i) with a concomitant loss of sustained phase. Furthermore, pretreatment of cells with dantrolene resulted in a decrease of the early response of [Ca2+](i), indicating the initial effect of the enterotoxin on the rise of [Ca2+](i) was mostly due to its mobilization from some IP3-sensitive intracellular stores.

引用

页码：131 / 133

页数：3

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