IMMUNOCYTOCHEMICAL LOCALIZATION OF NICOTINIC ACETYLCHOLINE-RECEPTOR IN RAT HYPOTHALAMUS

被引：35

作者：

OKUDA, H ^{[1
]}

SHIODA, S ^{[1
]}

NAKAI, Y ^{[1
]}

NAKAYAMA, H ^{[1
]}

OKAMOTO, M ^{[1
]}

NAKASHIMA, T ^{[1
]}

机构：

[1] SHOWA UNIV, SCH MED, DEPT ANAT 1, TOKYO 142, JAPAN

来源：

BRAIN RESEARCH | 1993年 / 625卷 / 01期

关键词：

ACETYLCHOLINE RECEPTOR; HYPOTHALAMUS; IMMUNOCYTOCHEMISTRY; NICOTINIC RECEPTOR; RAT;

D O I：

10.1016/0006-8993(93)90147-F

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Immunocytochemical localization of neuronal nicotinic acetylcholine receptor (nAChR) was examined in rat hypothalamus. Monoclonal antibody against alpha4 ACh-binding subunits of nAChR was used in the avidin-biotin-peroxidase complex (ABC) immunocytochemical method at both the light and electron microscopic levels. By light microscopy nAChR-like immunoreactivity was found in many neuronal cell bodies and their fibers in the paraventricular nucleus (PVN) and in many axons and axon terminals in the median eminence (ME). The immunoreactivity of nAChR was the most intense in the ME. By electron microscopy immunoreaction products occurred on the rough endoplasmic reticulum, nuclear envelope, cytoplasmic matrices and postsynaptic densities of synaptic junctions in some neurons in the parvocellular part of the PVN. In the external layer of the ME, nAChR-like immunoreactivity was found over the entire plasma membranes of many axon terminals. Involvement of nAChRs in the release of neurotransmitters and neuropeptides both in the PVN and the ME is discussed.

引用

页码：145 / 151

页数：7

共 38 条

[1] FUNCTIONAL EXPRESSION OF 2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS FROM CDNA CLONES IDENTIFIES A GENE FAMILY [J].