COMPLETE NUCLEOTIDE-SEQUENCE OF A CYTOPATHIC HEPATITIS-A VIRUS-STRAIN ISOLATED IN ITALY

被引：38

作者：

BENEDUCE, F

PISANI, G

DIVIZIA, M

PANA, A

MORACE, G

机构：

[1] IST SUPER SANITA,VIROL LAB,I-00161 ROME,ITALY

[2] UNIV ROMA TOR VERGATA,DEPT HYG,ROME,ITALY

来源：

VIRUS RESEARCH | 1995年 / 36卷 / 2-3期

关键词：

HAV-FG STRAIN; CYTOPATHIC EFFECT; NUCLEOTIDE SEQUENCE;

D O I：

10.1016/0168-1702(95)00009-F

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The molecular basis of the cytopathic effect induced in cell culture by some hepatitis A virus (HAV) strains and variants has not been determined. In order to assess the molecular mechanism(s) underlying this particular phenotype the genome of an Italian cytopathic isolate (strain FG) was sequenced from cDNAs obtained by RT-PCR. Sequence analysis revealed the presence of mutations common to either adapted or cytopathic variants of HAV. In particular, amino acid deletions in proteins VP1 and 3A were detected. Expression of protein 3A in E. coli showed that the N-terminal deletion renders this protein toxic to bacteria.

引用

页码：299 / 309

页数：11

共 35 条

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