ENHANCING THE ENANTIOSELECTIVITY OF CANDIDA LIPASE CATALYZED ESTER HYDROLYSIS VIA NONCOVALENT ENZYME MODIFICATION

被引：164

作者：

WU, SH ^{[1
]}

GUO, ZW ^{[1
]}

SIH, CJ ^{[1
]}

机构：

[1] UNIV WISCONSIN, SCH PHARM, MADISON, WI 53706 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1990年 / 112卷 / 05期

关键词：

D O I：

10.1021/ja00161a052

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

When the lipase of Candida cylindracea was treated with deoxycholate and organic solvents (ether-ethanol, 1:1), the enzyme apparently unfolded and refolded to generate a more stable conformer, termed lipase A-form. This lipase A-form was found to be considerably more enantioselective than the native enzyme toward the hydrolysis of a variety of (±)-arylpropionic and (±)-phenoxypropionic esters. © 1990, American Chemical Society. All rights reserved.

引用

页码：1990 / 1995

页数：6

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