INTERVENING SEQUENCES IN AN ARCHAEA DNA-POLYMERASE GENE

被引：173

作者：

PERLER, FB ^{[1
]}

COMB, DG ^{[1
]}

JACK, WE ^{[1
]}

MORAN, LS ^{[1
]}

QIANG, BQ ^{[1
]}

KUCERA, RB ^{[1
]}

BENNER, J ^{[1
]}

SLATKO, BE ^{[1
]}

NWANKWO, DO ^{[1
]}

HEMPSTEAD, SK ^{[1
]}

CARLOW, CKS ^{[1
]}

JANNASCH, H ^{[1
]}

机构：

[1] WOODS HOLE OCEANOG INST,WOODS HOLE,MA 02543

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 12期

关键词：

INTRON; SELF-SPLICING; PROTEIN SPLICING;

D O I：

10.1073/pnas.89.12.5577

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The DNA polymerase gene from the Archaea Thermococcus litoralis has been cloned and expressed in Escherichia coli. It is split by two intervening sequences (IVSs) that form one continuous open reading frame with the three polymerase exons. To our knowledge, neither IVS is similar to previously described introns. However, the deduced amino acid sequences of both IVSs are similar to open reading frames present in mobile group I introns. The second IVS (IVS2) encodes an endonuclease, I-Tli I, that cleaves at the exon 2-exon 3 junction after IVS2 has been deleted. IVS2 self-splices in E. coli to yield active polymerase, but processing is abolished if the IVS2 reading frame is disrupted. Silent changes in the DNA sequence at the exon 2-IVS2 junction that maintain the original protein sequence do not inhibit splicing. These data suggest that protein rather than mRNA splicing may be responsible for production of the mature polymerase.

引用

页码：5577 / 5581

页数：5

共 26 条

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