ACCUMULATION AND POLYSOMAL RECRUITMENT OF TRANSCRIPTS IN RESPONSE TO DESICCATION AND REHYDRATION OF THE MOSS TORTULA-RURALIS

被引:51
作者
SCOTT, HB [1 ]
OLIVER, MJ [1 ]
机构
[1] USDA ARS,CROPPING SYST RES LAB,PLANT STRESS GRP,LUBBOCK,TX 79401
关键词
DESICCATION TOLERANCE; RECOVERY; CDNA CLONES; POLYSOMAL RNA; TORTULA RURALIS;
D O I
10.1093/jxb/45.5.577
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In a continuing effort to understand vegetative desiccation tolerance in poikilohydric plants we have isolated cDNA clones that represent transcripts involved in the response of the desiccation-tolerant moss Tortula ruralis to desiccation and rehydration. These clones were used in Northern analyses to determine the expression characteristics of the represented transcripts following the desiccation and rehydration of the moss gametophytes. The clones were classified into three groups; hydration, recovery and constitutive, according to their recruitment into polysomal fractions in response to the treatment. The expression characteristics of these clones confirmed the hypothesis that the response of the moss to desiccation and rehydration involves an alteration in the control of translation (at least at the level of transcript recruitment into polysomes). However, this analysis also revealed that changes in the level of transcript accumulation plays an important role in the change in gene expression manifested upon rehydration.
引用
收藏
页码:577 / 583
页数:7
相关论文
共 24 条
[1]  
Ausubel F, 1988, CURRENT PROTOCOLS MO
[2]   A DESICCATION-RELATED ELIP-LIKE GENE FROM THE RESURRECTION PLANT CRATEROSTIGMA-PLANTAGINEUM IS REGULATED BY LIGHT AND ABA [J].
BARTELS, D ;
HANKE, C ;
SCHNEIDER, K ;
MICHEL, D ;
SALAMINI, F .
EMBO JOURNAL, 1992, 11 (08) :2771-2778
[3]  
BARTELS D, 1990, PLANTA, V181, P27, DOI 10.1007/BF00202321
[4]   ULTRASTRUCTURAL AND BIOCHEMICAL-BASES OF RESURRECTION IN THE DROUGHT-TOLERANT VASCULAR PLANT, SELAGINELLA-LEPIDOPHYLLA [J].
BERGTROM, G ;
SCHALLER, M ;
EICKMEIER, WG .
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1982, 78 (03) :269-282
[5]  
Bewley, 1984, ADV BRYOLOGY, V2, P91
[6]  
Bewley J. D., 1992, Water and life: comparative analysis of water relationships at the organismic, cellular and molecular level., P141
[7]  
BEWLEY J D, 1974, Royal Society of New Zealand Bulletin, V12, P395
[8]  
BEWLEY JD, 1993, IN PRESS 16TH P ANN
[9]  
BEWLEY JD, 1982, ENCY PLANT PHYSL B, V12, P325
[10]   LOW-MOLECULAR-WEIGHT SOLUTES IN DESICCATED AND ABA-TREATED CALLI AND LEAVES OF CRATEROSTIGMA-PLANTAGINEUM [J].
BIANCHI, G ;
GAMBA, A ;
MURELLI, C ;
SALAMINI, F ;
BARTELS, D .
PHYTOCHEMISTRY, 1992, 31 (06) :1917-1922