EFFECT OF WATER ACTIVITY ON THERMOLYSIN-CATALYZED PEPTIDE-SYNTHESIS IN ORGANIC-SOLVENTS

被引：11

作者：

HWANG, KA ^{[1
]}

LEE, SB ^{[1
]}

LEE, KH ^{[1
]}

机构：

[1] POHANG UNIV SCI & TECHNOL,DEPT CHEM ENGN,POHANG 790784,SOUTH KOREA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 01期

关键词：

D O I：

10.1007/BF00134199

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of water activity on the rate of thermolysin-catalyzed synthesis of an aspartame precursor has been investigated in water-miscible and water-immiscible solvents. In both cases, the enzyme reaction rate at a given water activity was found to be significantly different depending on the nature of the solvent. The reaction rates in water-immiscible solvents, where the water activities were close to 1.0, were found to be significantly dependent on the volume ratio of water to organic media and the hydrophobicity of the solvent. These data suggest that the enzyme reaction in the solvent is influenced appreciably by other factors in addition to the water activity.

引用

页码：71 / 76

页数：6

共 13 条

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