DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN TISSUE BLOCKS BY INSITU HYBRIDIZATION AS COMPARED WITH A POLYMERASE CHAIN-REACTION PROCEDURE

被引：20

作者：

SKYLDBERG, B

KALANTARI, M

KARKI, M

JOHANSSON, B

HAGMAR, B

WALAAS, L

机构：

[1] SAHLGRENS UNIV HOSP, DEPT CLIN PATHOL & CYTOL, S-41345 GOTHENBURG, SWEDEN

[2] STOCKHOLM COLL HLTH & CARING SCI, STOCKHOLM, SWEDEN

[3] STOCKHOLM CTY COUNCIL, CENT MICROBIOL LAB, DEPT VIROL, STOCKHOLM, SWEDEN

[4] SABBATSBERGS HOSP, DEPT PATHOL & CYTOL, S-11382 STOCKHOLM, SWEDEN

来源：

HUMAN PATHOLOGY | 1991年 / 22卷 / 06期

关键词：

HUMAN PAPILLOMAVIRUS; INSITU HYBRIDIZATION; POLYMERASE CHAIN REACTION; PARAFFIN SECTIONS;

D O I：

10.1016/0046-8177(91)90235-H

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Tissue blocks from 25 cases of condyloma and/or dysplasia were used for human papillomavirus typing with DNA in situ hybridization, compared with the very sensitive polymerase chain reaction. Only one of these cases was negative with both methods; a case of vaginal "koilocytosis". Polymerase chain reaction, as expected, was the more sensitive method, positive in 24 cases, with seven double infections. In situ hybridization was positive in 18 cases, with only two detected double infections. There was excellent agreement between the two methods in typing results. In all cases in situ hybridization showed a positive reaction in areas of koilocytosis and/or dysplasia. © 1991.

引用

页码：578 / 582

页数：5

共 19 条

[1] GENITAL HUMAN PAPILLOMAVIRUS INFECTIONS [J].