PATCHY ACCUMULATION OF APICAL NA+ TRANSPORTERS ALLOWS CROSS TALK BETWEEN EXTRACELLULAR-SPACE AND CELL-NUCLEUS

被引：14

作者：

OBERLEITHNER, H

WUNSCH, S

SCHNEIDER, S

机构：

[1] Department of Physiology, University of Würzburg, 8700 Würzburg

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 01期

关键词：

NA+ CHANNEL; GENE ACTIVATION; CELL FUSION;

D O I：

10.1073/pnas.89.1.241

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intracellular Na+ activities and local current densities were measured in fused Madin-Darby canine kidney cells using Na+ and voltage-sensing microelectrodes. Na+ that enters the cell across the apical plasma membrane accumulates initially in the nucleoplasm, several seconds ahead of its appearance in the cell cytoplasm. The spatial distribution of Na+ currents, produced by a local superfusion of the cell surface, indicates a nonuniform, patchy accumulation of apical Na+ transporters in the vicinity of the nucleus. Such pathways for direct Na+ flux between extracellular space and cell nucleus could be potentially important for gene activation.

引用

页码：241 / 245

页数：5

共 22 条

[1] LATERAL DISTRIBUTION OF SODIUM AND POTASSIUM CHANNELS IN FROG SKELETAL-MUSCLE - MEASUREMENTS WITH A PATCH-CLAMP TECHNIQUE [J].