CASTING FOR MULTICOMPONENT DNA-BINDING COMPLEXES

被引：46

作者：

WRIGHT, WE

FUNK, WD

机构：

[1] W. E. Wright and W. D. Funk are, the University of Texas Southwestern Medical Center, Dallas, TX 75235

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1993年 / 18卷 / 03期

关键词：

D O I：

10.1016/0968-0004(93)90156-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Degenerate oligonucleotides and polymerase chain reaction-based reiterative selection techniques have been used to define the consensus binding sites for an increasing number of transcription factors. The use of crude nuclear extracts rather than purified proteins permits multicomponent complexes to form, and allows the technique to generate information about the combinatorial interactions involved in gene regulation.

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收藏

页码：77 / 80

页数：4

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