REMOVAL OF PHENOLS FROM WASTE-WATER BY SOLUBLE AND IMMOBILIZED TYROSINASE

被引:106
作者
WADA, S [1 ]
ICHIKAWA, H [1 ]
TATSUMI, K [1 ]
机构
[1] NATL INST RESOURCES & ENVIRONM,16-3 ONOGAWA,TSUKUBA,IBARAKI 305,JAPAN
关键词
PHENOL; TYROSINASE; IMMOBILIZATION; CHITOSAN; IMMOBILIZED ENZYME;
D O I
10.1002/bit.260420710
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An enzymatic method for removal of phenols from industrial wastewater was investigated. Phenols in an aqueous solution were removed after treatment with mushroom tyrosinass. The reduction order of substituted phenols is catechol > p-cresol > p-chlorophenol > phenol > p-methoxyphenol. In the treatment of tyrosinase alone, no precipitate was formed but a color change from colorless to dark-brown was observed. The colored products were removed by chitin and chitosan which are available abundantly as shellfish waste. In addition, the reduction rate of phenols was observed to be accelerated in the presence of chitosan. Tyrosinass, immobilized by using amino groups in the enzyme on cation exchange resins, can be used repeatedly. By treatment with immobilized tyrosinase, 100% of phenol was removed after 2 h, and the activity was reduced very little even after 10 repeat treatments. (C) 1993 John Wiley & Sons, Inc.
引用
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页码:854 / 858
页数:5
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