RAPID DIAGNOSIS OF MYCOBACTERIUM-TUBERCULOSIS INFECTIONS BY AN ELISA-LIKE DETECTION OF POLYMERASE CHAIN-REACTION PRODUCTS

被引：31

作者：

ZAMBARDI, G ^{[1
]}

DRUETTA, A ^{[1
]}

ROURE, C ^{[1
]}

FOUQUE, B ^{[1
]}

GIRARDO, P ^{[1
]}

CHYPRE, C ^{[1
]}

MARCHAND, J ^{[1
]}

FRENEY, J ^{[1
]}

FLEURETTE, J ^{[1
]}

机构：

[1] CIS BIO INT,SONDES MOLEC LAB,F-91192 GIF SUR YVETTE,FRANCE

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 02期

关键词：

MYCOBACTERIUM TUBERCULOSIS; PCR; DNA PROBE; DIAGNOSIS;

D O I：

10.1016/S0890-8508(95)80033-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymerase chain reaction (PCR) assay was developed for the detection in clinical samples of mycobacteria belonging to the Mycobacterium tuberculosis complex. PCR products were detected with a simple and rapid colorimetric method. With this method, 50 fg of M. tuberculosis DNA were detectable with the repetitive DNA-sequence-derived primers, corresponding to 10 genome equivalents. Detection of M. tuberculosis in 258 clinical samples by PCR was compared with detection by culture. PCR was positive for 56 of 57 culture-positive and Ziehl-Neelsen-staining-positive (ZN) samples, 11 of 18 culture-positive and ZN-negative samples. The presence of groEL DNA sequences was also investigated by PCR for all the specimens with the same revelation protocol. Three of the eight false-negative samples with the repetitive element-derived primers were found to contain groEL DNA sequences specific for the Mycobacterium genus. Among the 183 culture-negative samples, 30 were positive by PCR. When clinical data were known, the diagnosis of tuberculosis was established for the patients from whom those samples had been obtained. The results show that the rapid and simplified PCR assay described here is slightly more sensitive than culture and can be used in routine clinical practice.

引用

页码：91 / 99

页数：9

共 28 条

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