TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY FOR HUMAN INTERFERON-GAMMA

被引：1

作者：

HARA, H

CHIKUMA, M

KOMINAMI, G

机构：

[1] SHIONOGI & CO LTD,SHIONOGI RES LABS,TOYONAKA,OSAKA 561,JAPAN

[2] OSAKA UNIV PHARMACEUT SCI,MATSUBARA,OSAKA 580,JAPAN

来源：

ANALYTICAL SCIENCES | 1995年 / 11卷 / 03期

关键词：

INTERFERON-GAMMA; TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY; IMMUNOENZYMOMETRIC ASSAY;

D O I：

10.2116/analsci.11.411

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A time-resolved immunofluorometric assay (TR-IFMA) for interferon-gamma (IFN-gamma) was developed and its properties were compared with those of previously reported immunoenzymometric assays (IEMAs). The advantage of TR-IFMA is not only that it is as sensitive as the usual colorimetric IEMA, but that it is also feasible and fast as a detection procedure of europium. It was modified in order to make it more sensitive and faster by changing the antiserum and incubation conditions. The improved assay could directly measure IFN-gamma in human plasma without any pretreatment. The detection limit for IFN-gamma is 40 pg/ml, and an assay can be completed within 6 h.

引用

页码：411 / 414

页数：4

共 12 条

[1] HUMAN INTERFERON-GAMMA QUANTIFIED VIA A SENSITIVE ONE-SITE MONOCLONAL-ANTIBODY IN A SANDWICH ELISA - A PEG MODIFICATION FOR MEASUREMENT IN SERUM [J].