EFFECTS OF ANTIGENS FROM AEROMONAS-SALMONICIDA SSP ACHROMOGENES ON LEUKOCYTES FROM PRIMED AND UNPRIMED ATLANTIC SALMON (SALMO-SALAR L)

被引：13

作者：

GUDMUNDSDOTTIR, S

MAGNADOTTIR, B

GUDMUNSDOTTIR, BK

机构：

[1] Institute for Experimental Pathology, University of Iceland, Keldur, IS-112, Reykjavík

来源：

FISH & SHELLFISH IMMUNOLOGY | 1995年 / 5卷 / 07期

关键词：

ATLANTIC SALMON; LEUKOCYTE PROLIFERATION ASSAY; AEROMONAS SALMONICIDA SSP ACHROMOGENES ANTIGENS;

D O I：

10.1016/S1050-4648(95)80050-6

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The effects of extracellular antigens isolated from Aeromonas salmonicida ssp. achromogenes, were tested on leukocyte cultures from the head-kidney of Atlantic salmon (Salmo salar L.) fingerlings. Samples from vaccinated fingerlings were compared to samples from control fingerlings that were injected with incomplete Freunds adjuvant (IFA) or phosphate buffered saline (PBS). The antigen preparations used for the comparative studies were extracellular products (ECP) and a 20 kDa toxic metallo-caseinase (AsaP1). Lipopolysaccharide (LPS) isolated from the bacterium was tested on unvaccinated fingerlings only. All antigen preparations were mitogenic to cultures from unvaccinated fish. In cultures from vaccinated fish there were difficulties in discerning between mitogenic effects, adjuvant induced cell activation and possible specific responses to the antigens. (C) 1995 Academic Press Limited

引用

页码：493 / 504

页数：12

共 29 条

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