MOLECULAR-CLONING OF THE COMPLEMENTARY-DNA FOR THE MOUSE PYRUVATE-KINASE M-2 GENE WHOSE EXPRESSION IS DEPENDENT UPON CELL-DIFFERENTIATION

被引：12

作者：

IZUMI, S ^{[1
]}

MANABE, A ^{[1
]}

TOMOYASU, A ^{[1
]}

KIHARANEGISHI, F ^{[1
]}

ARIGA, H ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1995年 / 1267卷 / 2-3期

关键词：

CDNA SEQUENCE; P19; CELL; DIFFERENTIATION; PYRUVATE KINASE;

D O I：

10.1016/0167-4889(95)00071-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding M2-type pyruvate kinase from mouse was cloned and its nucleotide sequence was determined. The cDNA encoded a protein containing 531 amino acids, the nucleotide and amino acid sequences of which were 93.2% and 98.1%, respectively, homologous to those of rat M2 pyruvate kinase. Expression of the pyruvate kinase in mouse embryonal carcinoma P19 cells altered according to differentiation stages; high at undifferentiated and low at differentiated stages.

引用

页码：135 / 138

页数：4

共 10 条

[1]

IMAMURA K, 1982, METHOD ENZYMOL, V90, P150

[2] BOX DNA - A NOVEL REGULATORY ELEMENT RELATED TO EMBRYONAL CARCINOMA CELL-DIFFERENTIATION [J].

KIHARANEGISHI, F ;

TSUJITA, R ;

NEGISHI, Y ;

ARIGA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (12) :7747-7756

[3] COMPLETE NUCLEOTIDE AND DEDUCED AMINO-ACID-SEQUENCES OF RAT L-TYPE PYRUVATE-KINASE [J].

LONE, YC ;

SIMON, MP ;

KAHN, A ;

MARIE, J .

FEBS LETTERS, 1986, 195 (1-2) :97-100

[4] ISOENZYMES OF PYRUVATE-KINASE [J].

MUIRHEAD, H .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1990, 18 (02) :193-196

[5]

NOGUCHI T, 1986, J BIOL CHEM, V261, P3807

[6]

NOGUCHI T, 1987, J BIOL CHEM, V262, P14336

[7]

Sambrook J., 1989, MOL CLONING LAB MANU

[8]

TANI K, 1988, GENE, V73, P509

[9]

TREMP GL, 1989, J BIOL CHEM, V264, P19904

[10]

YANAGI S, 1974, CANCER RES, V34, P2283

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