CLONING OF THE WIDE SPECTRUM AMIDASE GENE FROM BREVIBACTERIUM SP R312 BY GENETIC COMPLEMENTATION - OVEREXPRESSION IN BREVIBACTERIUM SP AND ESCHERICHIA-COLI

被引：4

作者：

AZZA, S ^{[1
]}

BIGEY, F ^{[1
]}

ARNAUD, A ^{[1
]}

GALZY, P ^{[1
]}

机构：

[1] ECOLE NATL SUPER AGRON MONTIPELLIER, CHAIR MICROBIOL IND & GENET MICOORGANISMES, F-34060 MONTPELLIER 1, FRANCE

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 122卷 / 1-2期

关键词：

BREVIBACTERIUM SP; AMIE GENE CLONING; COMPLEMENTATION AND EXPRESSION;

D O I：

10.1111/j.1574-6968.1994.tb07155.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The amiE gene of Brevibacterium sp. R312 encoding wide spectrum amidase was isolated by complementation of a Brevibacterium sp. mutant using a plasmid gene bank of chromosomal DNA. The amiE structural gene and its promoter were localized on a 1.8-kb fragment by subsequent subcloning and complementation studies. Another promoter localized in the pSR 1 fragment of the cloning vector was shown to be able to control amiE gene expression. In Brevibacterium sp., the investigation of amidase activities related to one copy of the gene suggested that the regulation of the amiE gene expression was under negative control. High expression levels have been obtained in Brevibacterium sp. and, after substitution of the amiE promoter by the tac promoter, in Escherichia coli.

引用

页码：129 / 136

页数：8

共 23 条

[1] THE DNA-SEQUENCE AND MINIMAL REPLICON OF THE CORYNEBACTERIUM-GLUTAMICUM PLASMID PSR1 - EVIDENCE OF A COMMON ANCESTRY WITH PLASMIDS FROM C-DIPHTHERIAE [J].