VISUALIZATION OF RNA TERTIARY STRUCTURE BY RNA-EDTA-CENTER-DOT-FE(II) AUTOCLEAVAGE - ANALYSIS OF TRNA(PHE) WITH URIDINE-EDTA-CENTER-DOT-FE(II) AT POSITION-47

被引：50

作者：

HAN, HY

DERVAN, PB

机构：

[1] Beckman Institute, California Institute of Technology, Pasadena

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 11期

关键词：

AFFINITY CLEAVING; YEAST TRNA(PHE); CLEAVAGE OF RNA;

D O I：

10.1073/pnas.91.11.4955

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA.Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)(2)(SO4)(2) and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

引用

页码：4955 / 4959

页数：5

共 35 条

[1] LEAD-CATALYZED CLEAVAGE OF YEAST TRANSFER RNAPHE MUTANTS
BEHLEN, LS
SAMPSON, JR
DIRENZO, AB
UHLENBECK, OC
[J]. BIOCHEMISTRY, 1990, 29 (10) : 2515 - 2523
[2] CRYSTALLOGRAPHIC AND BIOCHEMICAL INVESTIGATION OF THE LEAD(II)-CATALYZED HYDROLYSIS OF YEAST PHENYLALANINE TRANSFER-RNA
BROWN, RS
DEWAN, JC
KLUG, A
[J]. BIOCHEMISTRY, 1985, 24 (18) : 4785 - 4801
[3] DECH TR, 1990, ANNU REV BIOCHEM, V59, P543
[4] DESIGN OF SEQUENCE-SPECIFIC DNA-BINDING MOLECULES
DERVAN, PB
[J]. SCIENCE, 1986, 232 (4749) : 464 - 471
[5] THE RNA-FOLDING PROBLEM
DRAPER, DE
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1992, 25 (04) : 201 - 207
[6] SEQUENCE-SPECIFIC CLEAVAGE OF SINGLE-STRANDED-DNA - OLIGODEOXYNUCLEOTIDE-EDTA.FE(II)
DREYER, GB
DERVAN, PB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (04) : 968 - 972
[7] 3'-TERMINAL LABELING OF RNA WITH T4 RNA LIGASE
ENGLAND, TE
UHLENBECK, OC
[J]. NATURE, 1978, 275 (5680) : 560 - 561
[8] Gait M. J, 1984, OLIGONUCLEOTIDE SYNT
[9] STRUCTURE OF AN UNMODIFIED TRANSFER-RNA MOLECULE
HALL, KB
SAMPSON, JR
UHLENBECK, OC
REDFIELD, AG
[J]. BIOCHEMISTRY, 1989, 28 (14) : 5794 - 5801
[10] SEQUENCE-SPECIFIC RECOGNITION OF DOUBLE HELICAL RNA AND RNA.DNA BY TRIPLE-HELIX FORMATION
HAN, HY
DERVAN, PB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (09) : 3806 - 3810

← 1 2 3 4 →