Induction and activation of procollagenase in rabbit synovial fibroblasts after treatment with active oxygen released by xanthine xanthine oxidase

被引：8

作者：

Ehrlich, W ^{[1
]}

Huser, H ^{[1
]}

Kroger, H ^{[1
]}

机构：

[1] ROBERT KOCH INST,D-13353 BERLIN,GERMANY

来源：

RHEUMATOLOGY INTERNATIONAL | 1995年 / 15卷 / 04期

关键词：

synovial fibroblasts; collagenase; active oxygen; poly(ADP-ribosyl)ation;

D O I：

10.1007/BF00301769

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Treatment of rabbit synovial fibroblasts with active oxygen (AO) released by xanthine/xanthine oxidase resulted in an induction of procollagenase in these cells in concentrations ranging from 12.5 mu g/ml xanthine plus 0.0025 U/ml xanthine oxidase to 50 mu g/ml xanthine plus 0.01 U/ml xanthine oxidase. Preceding this there was an accumulation of poly(ADP-ribose) for the same concentration range of xanthine/xanthine oxidase. Furthermore, it was found that AO caused activation of the latent procollagenase to the active enzyme in concentrations ranging from 0.1 mu g/ml xanthine plus 0.00002 U/ml xanthine oxidase to 1 mu g/ml xanthine plus 0.0002 U/ml xanthine oxidase. It is suggested that poly(ADP-ribosyl)ation participates in the induction of procollagenase by relaxing chromatin. Furthermore, it is proposed that AO activates latent procollagenase under physiological conditions.

引用

页码：131 / 136

页数：6

共 38 条

[1] POLY(ADP-RIBOSE) CATABOLISM IN MAMMALIAN-CELLS EXPOSED TO DNA-DAMAGING AGENTS [J].