ISOLATION AND CHARACTERIZATION OF 2 MALE-SPECIFIC DNA FRAGMENTS FROM THE BOVINE GENE

被引：52

作者：

MILLER, JR

KOOPMAN, M

机构：

[1] Department of Molecular Embryology, Institute of Animal Physiology and Genetics Research, Cambridge, Babraham

来源：

ANIMAL GENETICS | 1990年 / 21卷 / 01期

关键词：

cattle; male‐specific; Y chromosome;

D O I：

10.1111/j.1365-2052.1990.tb03209.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Summary Two independent male‐specific DNA fragments were isolated from the bovine genome by the technique of cloning by deletion enrichment. Each is represented in the order of one to five copies per haploid genome. Neither fragment was found to be polymorphic between two individuals of different breeds with 12 different restriction enzymes. A ‘zoo blot’ demonstrated that one fragment had a closely related sequence in the sheep genome, although no other hybridizing sequences were detected in a variety of animal DNAs tested with both probes. The DNA sequence of each fragment is presented; one fragment has a continuous open reading frame and a potential Z‐DNA forming region. 1990 International Society for Animal Genetics

引用

页码：77 / 82

页数：6

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