CLONING AND CHARACTERIZATION OF A DNA FRAGMENT THAT COMPLEMENTS THE NFXB MUTATION IN PSEUDOMONAS-AERUGINOSA PAO

被引：17

作者：

OKAZAKI, T

IYOBE, S

HASHIMOTO, H

HIRAI, K

机构：

[1] KYORIN PHARMACEUT CO LTD, CENT RES LABS, 2399-1 NOGI MACHI, TOCHIGI 32901, JAPAN

[2] GUNMA UNIV, SCH MED, DEPT MICROBIOL, MAEBASHI, GUNMA 371, JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 79卷 / 01期

关键词：

PSEUDOMONAS-AERUGINOSA; NEW QUINOLONE ANTIMICROBIAL AGENT; NFXB; MEMBRANE PERMEABILITY; CLONING;

D O I：

10.1016/0378-1097(91)90522-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 2.2-kilobase DNA fragment that can complement the nfxB mutation in Pseudomonas aeruginosa PAO was cloned from chromosomal DNA of P. aeruginosa PAO1. The nfxB mutants show an increase in resistance to quinolones and hypersusceptibility to beta-lactam and aminoglycoside antibiotics. In the mutants, a 54 kDa outer membrane protein newly appeared. The phenotype was restored to the wild-type by transformation with plasmid carrying the 2.2-kb DNA fragment.

引用

页码：31 / 35

页数：5

共 19 条

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