NEW TETRAZOLE-1-ACETIC ACID-ESTERS FOR ENZYMATIC-SYNTHESIS OF CEFAZOLIN

被引：16

作者：

KOSTADINOV, M ^{[1
]}

NIKOLOV, A ^{[1
]}

TSONEVA, N ^{[1
]}

PETKOV, N ^{[1
]}

机构：

[1] ANTIBIOT LTD,ANTIBIOT RES INST,BU-7200 RAZGRAD,BULGARIA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1992年 / 33卷 / 03期

关键词：

ENZYMATIC SYNTHESIS; PENICILLINACYLASE; CEPHALOSPORINS; CEFAZOLIN; TETRAZOLE-1-ACETIC ACID;

D O I：

10.1007/BF02921833

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymatic synthesis of cefazolin (CEZ) using esters of tetrazole-1-acetic acid (TzAA esters) with saturated lower alcohols is reported. The optimum ratios of acyl-donor:acyl-acceptor in the enzymatic synthesis were determined. It is shown that a threefold molar excess of acyl-donor for about 165 min, a conversion rate of about 55% is obtained with these TzAA esters. The syntheses were carried out with commercial immobilized penicillinamidase/E.C.3.5.1.11/(Eupergit PcA) in a batch-type reactor. After 40 batches, the enzyme activity loss was 5-7% toward the initial one.

引用

页码：177 / 182

页数：6

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