MOLECULAR-CLONING, EXPRESSION, AND CHARACTERIZATION OF A 5TH MELANOCORTIN RECEPTOR

被引：275

作者：

GANTZ, I

SHIMOTO, Y

KONDA, Y

MIWA, H

DICKINSON, CJ

YAMADA, T

机构：

[1] UNIV MICHIGAN,MED CTR,DEPT INTERNAL MED,ANN ARBOR,MI 48109

[2] UNIV MICHIGAN,MED CTR,DEPT PEDIAT,ANN ARBOR,MI 48109

[3] UNIV MICHIGAN,MED CTR,DEPT PHYSIOL,ANN ARBOR,MI

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 200卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.1580

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the isolation of a gene encoding a novel member of the family of melanocortin receptors. The mouse melanocortin-5 receptor (mMC5R) responds to melanocortins with an increase in intracellular cyclic 3', 5'-adenosine monophosphate (cAMP) concentrations. Stimulation of the mMC5R by the melanocortins revealed a hierarchy of potency in which alpha-melanocyte stimulating hormone (alpha-MSH) > beta-melanocyte stimulating hormone (beta-MSH) > adrenocorticotropic hormone (ACTH) > gamma-melanocyte stimulating hormone (gamma-MSH). Further structure-activity studies indicated that amino- and carboxyl-terminal portions of alpha-MSH appear to be key determinants in the activation of mMC5R whereas the melanocortin core heptapeptide sequence is devoid of pharmacological activity. Northern blot analysis demonstrated the expression of mMC5R mRNA in mouse skeletal. muscle, lung, spleen, and brain. (C) 1994 Academic Press, Inc.

引用

页码：1214 / 1220

页数：7

共 20 条

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