A HIGHLY SPECIFIC AND SENSITIVE ENZYME-IMMUNOMETRIC ASSAY FOR CALCITONIN GENE-RELATED PEPTIDE BASED ON ENZYME AMPLIFICATION

被引：15

作者：

SETH, R

ZAIDI, M

FULLER, JQ

SELF, CH

机构：

[1] ROYAL POSTGRAD MED SCH, DEPT CHEM PATHOL, ENDOCRINE UNIT, DU CANE RD, LONDON W12 ONN, ENGLAND

[2] NOVO BIOLABS LTD, CAMBRIDGE CB2, ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1988年 / 111卷 / 01期

关键词：

D O I：

10.1016/0022-1759(88)90053-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Calcitonin gene-related peptide (CGRP) is an important member of the peptide family encoded by the calcitonin gene. It has been found to be a potent vasodilator in man and a major circulating gene product (Girgis et al., 1985). The present study reports the development of a sensitive and rapid two-site immunoassay for CGRP based on enzyme amplification (Self, 1985). The assay has been easy to construct, taking advantage of available antisera raised for other purposes. Nevertheless it has been found to be clearly superior to our previous radioimmunoassay in terms of sensitivity, specificity, speed and convenience.

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页码：11 / 16

页数：6

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