POLYSTYRENE, POLY-L-LYSINE AND NYLON AS ADSORPTIVE SURFACES FOR THE BINDING OF WHOLE CELLS OF MYCOBACTERIUM-TUBERCULOSIS H37 RV TO ELISA PLATES

被引：9

作者：

VERSCHOOR, JA ^{[1
]}

MEIRING, MJ ^{[1
]}

VANWYNGAARDT, S ^{[1
]}

WEYER, K ^{[1
]}

机构：

[1] S AFRICAN MRC,TB RES INST,PRETORIA 0001,SOUTH AFRICA

来源：

JOURNAL OF IMMUNOASSAY | 1990年 / 11卷 / 04期

关键词：

ELISA; Mycobacterium tuberculosis; nylon; poly-L-lysine; serodiagnosis;

D O I：

10.1080/01971529008055042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several methods of coating whole cells of Mycobacterium tu berculosis H37 RV to ELISA microtitre plates were compared with the aim of developing an ELISA screening assay for murine monoclonal antibodies in culture supernatants and human antibodies in patient sera. Undercoats of nylon or poly-L-lysine were compared to polystyrene as adsorptive surfaces for the bacteria, the effect of increased ionic strength and inclusion of SDS in the coating buffer measured, and methanol (70%) and glutaraldehyde (5%) investigated for their efficiency as fixatives of the bacterial monolayers. The results suggest PBS as a satisfactory coating buffer for the bacterial cells on polystyrene, and 70% methanol the preferred fixative for the dried antigen-coated plates. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：413 / 428

页数：16

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