THE EFFECT OF THE 13 DIP-ALLATOSTATINS ON MYOGENIC AND INDUCED CONTRACTIONS OF THE COCKROACH (DIPLOPTERA-PUNCTATA) HINDGUT

被引：118

作者：

LANGE, AB ^{[1
]}

BENDENA, WG ^{[1
]}

TOBE, SS ^{[1
]}

机构：

[1] QUEENS UNIV,DEPT BIOL,KINGSTON,ON K7L 3N6,CANADA

来源：

JOURNAL OF INSECT PHYSIOLOGY | 1995年 / 41卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

ALLATOSTATINS; HINDGUT CONTRACTIONS; DIPLOPTERA PUNCTATA; STRUCTURE ACTIVITY;

D O I：

10.1016/0022-1910(95)00008-I

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The Dip-allatostatins are a group of 13 neuropeptides, ranging in size from 6 to 18 amino acids, identified from a cloned cDNA sequence of Diploptera punctata, All 13 Dip-allatostatins inhibited both myogenic and proctolin-induced contractions of the hindgut of D, punctata. Dip-allatostatins were capable of reducing a 5 x 10(-6) M proctolin-induced contraction in a dose-dependent manner, with ED(50) values ranging from 1.7 x 10(-7) M for Dip-allatostatin 7 to greater than 1.3 x 10(-5) M for Dip-allatostatin 12, The effects of the Dip-allatostatins were reversible upon washing with saline, The free carboxylic acid (non-amidated) form of Dip-allatostatin 11, callatostatin 3 and callatostatin 5 showed a loss of inhibitory activity, In contrast to the inhibitory actions of the Dip-allatostatins on hindgut contractions, selected Dip-allatostatins (Dip-allatostatins 4, 6, 7, 10, 12), at concentrations ranging from 10(-7) M to 2 x 10(-5) M, did not alter the frequency or amplitude of myogenic contractions or inhibit proctolin-induced contractions of the cockroach oviduct muscle, Dip-allatostatins (Dip-allatostatins 1, 2, 4, 5, 6, 7) also had no effect on either myogenic or proctolin-induced contractions of the oviduct of Locusta migratoria.

引用

页码：581 / 588

页数：8

共 26 条

[1] ISOLATION OF PROCTOLIN, A MYOTROPIC PEPTIDE, FROM PERIPLANETA-AMERICANA [J].