PURIFICATION, CHARACTERIZATION, AND SOME PROPERTIES OF A VIRUS FROM THE APHID SITOBION-AVENAE

被引：7

作者：

ALLEN, MF

BALL, BV

机构：

[1] Department of Entomology and nematology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1990年 / 55卷 / 02期

关键词：

cereal aphids; characterization; incidence; isolation; Metopolophium dirhodum; nonoccluded virus; Sitobion (Macrosiphum) avenae; transmission;

D O I：

10.1016/0022-2011(90)90051-7

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

A new insect virus was isolated from the English grain aphid Sitobion (Macrosiphum) avenae and the rose grain aphid Metopolophium dirhodum maintained in continuous laboratory culture. The virus particles were isometric with a diameter of 30 nm. The particles from S. avenae had a sedimentation coefficient of 156 S, had a buoyant density of 1.366 in CsCl, contained RNA, and had one capsid protein of Mr 29,000. The virus was serologically unrelated to two similar viruses isolated from aphids-Rhopalosiphum padi virus and aphid lethal paralysis virus. It did not multiply in crop plants on which both S. avenae and M. dirhodum feed or in pupae of the honey bee, Apis mellifera, and was not detected in field-collected aphids. Virus could be transmitted to healthy aphids by contact with honeydew secreted by virus-infected individuals. © 1990.

引用

页码：162 / 168

页数：7

共 18 条

[1]

Adams J.B., 1972, P47

[2] 3 PREVIOUSLY UNDESCRIBED VIRUSES FROM HONEY BEE [J].

BAILEY, L ;

WOODS, RD .

JOURNAL OF GENERAL VIROLOGY, 1974, 25 (NOV) :175-186

[3] 2 MORE SMALL RNA VIRUSES FROM HONEY BEES AND FURTHER OBSERVATIONS ON SACBROOD AND ACUTE BEE-PARALYSIS VIRUSES [J].

BAILEY, L ;

WOODS, RD .

JOURNAL OF GENERAL VIROLOGY, 1977, 37 (OCT) :175-182

[4]

BAILEY L, 1981, HONEY BEE PATHOLOGY, P1

[5] PURIFICATION AND CHARACTERIZATION OF A VIRUS FROM THE APHID RHOPALOSIPHUM-PADI [J].

DARCY, CJ ;

BURNETT, PA ;

HEWINGS, AD ;

GOODMAN, RM .

VIROLOGY, 1981, 112 (01) :346-349

[6] DETECTION, BIOLOGICAL EFFECTS, AND TRANSMISSION OF A VIRUS OF THE APHID RHOPALOSIPHUM-PADI [J].

DARCY, CJ ;

BURNETT, PA ;

HEWINGS, AD .

VIROLOGY, 1981, 114 (01) :268-272

[7]

GUTTERIDGE RA, 1982, 1982 ROTH EXPT STA 1, P191

[8] ISOMETRIC, VIRUSLIKE PARTICLES IN GREEN PEACH APHID, MYZUS-PERSICAE - THEIR INTRACELLULAR-LOCALIZATION AND DISTRIBUTION WITHIN APHID BODY [J].

KITAJIMA, EW .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1976, 28 (01) :1-10

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] SLIDE GEL DIFFUSION PRECIPITIN TEST [J].

MANSI, W .

NATURE, 1958, 181 (4618) :1289-1290

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