A METHYLSULFONYL METABOLITE OF A POLYCHLORINATED BIPHENYL CAN SERVE AS A LIGAND FOR LIVER FATTY-ACID BINDING-PROTEIN IN RAT INTESTINAL-MUCOSA

被引：25

作者：

LARSEN, GL

BERGMAN, A

WEHLER, EK

BASS, NM

机构：

[1] UNIV STOCKHOLM,WALLENBERG LAB,S-10691 STOCKHOLM,SWEDEN

[2] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT MED,SAN FRANCISCO,CA 94143

[3] UNIV CALIF SAN FRANCISCO,SCH MED,CTR LIVER,SAN FRANCISCO,CA 94143

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1991年 / 77卷 / 03期

关键词：

4,4'-BIS([H-3]METHYLSULFONYL)-2,2',5,5'-TETRACHLOROBIPHENYL; FATTY-ACID-BINDING PROTEIN (FABP); INTESTINAL MUCOSA; BINDING PROTEIN; TOXICITY;

D O I：

10.1016/0009-2797(91)90040-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When a 100 000 x g supernatant from rat intestinal mucosa was incubated with 4,4'-bis([H-3]methylsulfonyl)-2,2',5,5'-tetrachlorobiphenyl, [(CT3SO2)2TCB] a (CT3SO2)2TCB-protein complex was formed. The (CT3SO2)2TCB-protein complex was isolated and purified using gel filtration and ion-exchange chromatography. The protein portion of this complex was characterized to be liver fatty acid binding protein (L-FABP) by SDS-polyacrylamide gel electrophoresis and immunoblot analysis. No cross reactivity was observed in the immunoblot analysis between the purified protein and anti-heart or anti-intestinal fatty acid binding protein. (CT3SO2)2TCB was extractable from L-FABP and therefore not covalently bound to L-FABP.

引用

页码：315 / 323

页数：9

共 29 条

[1] SPATIAL DIFFERENCES AND TEMPORAL TRENDS OF ORGANOCHLORINE COMPOUNDS IN BIOTA FROM THE NORTHWESTERN HEMISPHERE [J].