PREPARATIVE HPLC OF SOYBEAN TRYPSIN-INHIBITOR USING LARGE PARTICLE DIAMETER SUPPORTS

Two systems were developed to purify Soybean Trypsin Inhibitor (STI) using anion exchange chromatography. Both systems demonstrated that large diameter particles (30 and 55 mu m) could be used effectively for protein purification. Preparative samples of 455 mg and 7. 2 g were processed to yield highly purified products. Preparative purifications were achieved either on an analytical instrument with an analytical (0. 46 multiplied by 30 cm) column or on a large scale system with a preparative (4. 8 multiplied by 50 cm) column.