POLYPEPTIDE COMPOSITION OF THE 8S FORM OF PROLYL-TRANSFER-RNA SYNTHETASE FROM RAT-LIVER

被引：2

作者：

BIANCHI, C ^{[1
]}

PEREGO, R ^{[1
]}

DELMONTE, U ^{[1
]}

机构：

[1] CNR, CTR RES CELLULAR PATHOL, I-20133 MILAN, ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 187卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91306-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rat liver Fraction X containing the 24S complex of nine aminoacyl-tRNA synthetases, including prolyl-tRNA synthetase, was centrifuged on a 15-35% sucrose density gradient to obtain the 8S form of prolyl-tRNA synthetase. The enzyme was purified on a prolyldiaminohexyl-Sepharose 4B affinity column, specifically binding prolyl-tRNA synthetase to Sepharose-bound proline. After SDS-polyacrylamide gel electrophoresis, two peptides of 58 and 61 kDa were detected in the peak of prolyl-tRNA synthetase activity eluted from the affinity column. The 58 and 61 kDa peptides were also present in the 24S complex containing prolyl-tRNA synthetase activity isolated on the sucrose density gradient. © 1992.

引用

页码：1071 / 1076

页数：6

共 24 条

[1]

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[2]

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[3]

BIANCHI C, 1990, CELL BIOL INT REP S, P14

[4]

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