MGM101, A NUCLEAR GENE INVOLVED IN MAINTENANCE OF THE MITOCHONDRIAL GENOME IN SACCHAROMYCES-CEREVISIAE

被引:52
作者
CHEN, XJ [1 ]
GUAN, MX [1 ]
CLARKWALKER, GD [1 ]
机构
[1] AUSTRALIAN NATL UNIV, RES SCH BIOL SCI, MOLEC & POPULAT GENET GRP, GPO BOX 475, CANBERRA, ACT 2601, AUSTRALIA
关键词
D O I
10.1093/nar/21.15.3473
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A nuclear mutation, mgm101, results in temperature sensitive loss of mitochondrial DNA (mtDNA) in the yeast Saccharomyces cerevisiae. The corresponding gene, MGM101, was isolated from a genomic DNA library by complementation. Sequence analysis shows that MGM101 encodes a positively charged protein of 269 amino acids with a calculated molecular weight of 30 kDa. This analysis also reveals that MGM101 is adjacent to the ribosomal protein gene RPS7A on chromosome X and hybridization indicates it occurs in single copy. Creation of a null mutant by targeted disruption showed that the gene has no essential cellular function, aside from its participation in mitochondrial genome maintenance. As no counterpart has been identified in databases it is a novel protein whose role has yet to be determined. Expression of MGM101 is low on glucose medium but on galactose there is a two-fold increase in the level of the transcript.
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页码:3473 / 3477
页数:5
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