ENZYMATIC PROFILING OF IMMOBILIZED CELLS USING CZE

被引：17

作者：

MILLER, KJ ^{[1
]}

LYTLE, FE ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 14期

关键词：

D O I：

10.1021/ac00086a033

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Baker's yeast cells (Saccharomyces cerevisiae) were immobilized on a CZE capillary using a midcapillary frit of novel design. The frit was constructed using a single 50-mu m particle with ''through pores''. Cells trapped on the capillary were profiled using different amino acid beta-naphthylamide-aminopeptidase substrates. These substrates were introduced sequentially and metabolized for 1 min. After incubation the hydrolysis products were withdrawn by electrophoresis and detected by laser-induced fluorescence. An aminopeptidase profile was produced using just 500 cells. The new CZE-based method was found to have several significant advantages over the older cuvette/fluorometer method.

引用

页码：2420 / 2423

页数：4

共 18 条

[1] PERFUSION CHROMATOGRAPHY PACKING MATERIALS FOR PROTEINS AND PEPTIDES [J].