DETERMINATION OF AMINO-ACIDS IN BIOMASS AND PROTEIN SAMPLES BY MICROWAVE HYDROLYSIS AND ION-EXCHANGE CHROMATOGRAPHY

被引：32

作者：

JOERGENSEN, L

THESTRUP, HN

机构：

[1] DB Lab, Dansk Bioprotein A/S, 5230 Odense M

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 706卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)01107-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast method for protein hydrolysis based on controlled heating in a microwave oven is described. The samples are heated to 150 degrees C for 10-30 min where conventional methods use 110 degrees C for 24 h. The method was tested with pure protein samples and ''real'' protein samples with carbohydrates, fats, nucleic acids and minerals. The microwave method showed similar or better results than the conventional method. The effect of degassing and stabilising agents was also tested. Degassing and thioglycolic acid stabilise methionine and partly tryptophan. Degassing and phenol stabilise tyrosine, phenylalanine and histidine in performic acid oxidised samples.

引用

页码：421 / 428

页数：8

共 13 条

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[2]

Hunt S, 1985, CHEM BIOCH AMINO ACI, P376, DOI [10.1007/978-94-009-4832-7_12, DOI 10.1007/978-94-009-4832-712]

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