PEPTIDE SEQUENCE INFORMATION DERIVED BY PARTIAL ACID-HYDROLYSIS AND MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS-SPECTROMETRY

被引：51

作者：

VORM, O ^{[1
]}

ROEPSTORFF, P ^{[1
]}

机构：

[1] ODENSE UNIV, DEPT MOLEC BIOL, DK-5230 ODENSE, DENMARK

来源：

BIOLOGICAL MASS SPECTROMETRY | 1994年 / 23卷 / 12期

关键词：

D O I：

10.1002/bms.1200231204

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Partial acid hydrolysis of purified peptides followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of the resulting peptide mixture is a fast and simple procedure to confirm the identity of a peptide tentatively identified by its determined molecular weight. Often partial amino acid sequence information can be obtained directly. The sensitivity of the technique is in the high-femtomole to low-picomole range.

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页码：734 / 740

页数：7

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