INHIBITION OF REPLICATION OF HIV-1 BY RETROVIRAL VECTORS EXPRESSING TAT-ANTISENSE AND ANTI-TAT RIBOZYME RNA

被引:108
作者
LO, KMS
BIASOLO, MA
DEHNI, G
PALU, G
HASELTINE, WA
机构
[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV HUMAN RETROVIROL,44 BINNEY ST,BOSTON,MA 02115
[2] UNIV PADUA,INST MICROBIOL,I-35100 PADUA,ITALY
关键词
D O I
10.1016/0042-6822(92)91203-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A ribozyme was constructed that specifically cleaves RNA that contains the first coding exon of the tat gene of HIV-1. This anti-tat ribozyme was incorporated into a Moloney murine leukemia virus vector. A sequence containing only the 48-nucleotide antisense region of the ribozyme was also inserted into the retroviral vector. Human T-cell lines constitutively producing the tat-antisense and the anti-tat ribozyme RNA were created by transduction into Jurkat cells. When challenged with HIV-1, both the tat-antisense and anti-tat ribozyme-producing cells inhibited the replication of HIV-1. The antisense vector conferred a greater resistance to HIV-1 replication than did the anti-tat ribozyme vector. © 1992.
引用
收藏
页码:176 / 183
页数:8
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