GENOMIC ORGANIZATION OF THE MYCOBACTERIAL SIGMA-GENE-CLUSTER

被引：78

作者：

DOUKHAN, L

PREDICH, M

NAIR, G

DUSSURGET, O

MANDICMULEC, I

COLE, ST

SMITH, DR

SMITH, I

机构：

[1] PUBL HLTH RES INST, DEPT MICROBIOL, NEW YORK, NY 10016 USA

[2] UNIV PARIS 07, DEPT MICROBIOL, F-75251 PARIS 05, FRANCE

[3] ROCKEFELLER UNIV, CELLULAR PHYSIOL & IMMUNOL LAB, NEW YORK, NY 10021 USA

[4] INST PASTEUR, GENET MOLEC BACTERIENNE LAB, F-75724 PARIS 15, FRANCE

[5] COLLABORAT RES INC, WALTHAM, MA 01254 USA

来源：

GENE | 1995年 / 165卷 / 01期

关键词：

D O I：

10.1016/0378-1119(95)00427-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have previously described sigma(A) and sigma(B) and their structural genes, mysA and mysB, respectively, in Mycobacterium smegmatis. We have now sequenced the corresponding regions in the M. tuberculosis and M. leprae chromosomes, and have found the two homologous genes. The chromosomal linkage and the deduced amino acid (aa) sequences of the two genes show very high similarity in the three species of mycobacteria. We also report the finding of two other open reading frames (ORF) in these clusters. orfX, which has an unknown function, is located between mysA and mysB. The other ORF, located downstream from mysB, encodes a homolog of DtxR, the iron regulatory protein from Corynebacterium diphtheriae (Cd).

引用

页码：67 / 70

页数：4

共 13 条

[1]

Boyd, Oza, Murphy, Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae, Proc. Natl. Acad. Sci. USA, 87, pp. 5968-5972, (1990)

[2]

Dombroski, Walter, Gross, N-terminal amino acids modulate σ factor DNA-binding activity, Genes Dev., 7, pp. 2446-2455, (1993)

[3]

Eigelmeier, Honore, Woods, Caudron, Cole, Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae, Mol. Microbiol., 7, pp. 197-206, (1993)

[4]

Lonetto, Gribskov, Gross, The σ<sup>70</sup> family: sequence conservation and evolutionary relationships, J. Bacteriol., 174, pp. 3843-3849, (1992)

[5]

Oguiza, Tao, Marcos, Martin, Murphy, Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR homolog from Brevibacterium lactofermentum, J. Bacteriol., 177, pp. 465-467, (1995)

[6]

Predich, Doukhan, Nair, Smith, Characterization of RNA polymerase and two σ factor genes from Mycobacterium smegmatis, Mol. Microbiol., 15, pp. 355-366, (1995)

[7]

Qiu, Verlinde, Zhang, Schmitt, Holmes, Hol, Three-dimensional structure of the diphtheria toxin repressor in complex with divalent cation co-repressors, Structure, 3, pp. 87-100, (1995)

[8]

Schmitt, Holmes, Characterization of a defective diphtheria toxin repressor dtxR allele and analysis of dtxR transcription in wild-type and mutant strains of Corynebacterium diphtheriae, Infect. Immun., 59, pp. 3903-3908, (1991)

[9]

Schmitt, Predich, Doukhan, Smith, Holmes, Characterisation of the DtxR homolog from Mycobacterium tuberculosis, Infect. Immun., (1995)

[10]

Tanaka, Shiina, Takahashi, Nucleotide sequence of genes hrdA, hrdC, and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes, Mol. Gen. Genet., 229, pp. 334-340, (1991)

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