INTERGENERIC HYBRIDIZATION BETWEEN BRASSICA-NAPUS AND SINAPIS-PUBESCENS, AND THE CYTOLOGY AND CROSSABILITY OF THEIR PROGENIES

The cytological possibility of gene transfer from Sinapis pubescens to Brassica napus was investigated. Intergeneric hybrids between Brassica napus (2n = 38) and Sinapis pubescens (2n = 18) were produced through ovary culture. The F-1 hybrids were dihaploid and the chromosome configurations were (0-1)(III) + (2-11)(II) + (5-24)(I). One F-2 plant with 38 chromosomes was obtained from open pollination of the F-1 hybrid. Thirty-one seeds were obtained from the backcross of the F-2 plant with B. napus. Five out of seven plants had 38 chromosomes, and the pollen stainability ranged from 0% to 81.4%. In the B-2 plants obtained from the backcross of B, plants with B. napus, 66.7% of the plants examined had 38 chromosomes. S. pubescens may become a gene source for the improvement of B. napus.