THE TETRASACCHARIDE L-ALPHA-D-HEPTOSE1-]2-L-ALPHA-D-HEPTOSEL-]3-L-ALPHA-D-HEPTOSE1-](3-DEOXY-D-MANNO-OCTULOSONIC ACID) AND PHOSPHATE IN LIPID-A DEFINE THE CONSERVED EPITOPE IN HAEMOPHILUS LIPOPOLYSACCHARIDES RECOGNIZED BY A MONOCLONAL-ANTIBODY

被引:10
作者
BORRELLI, S
HEGEDUS, O
SHAW, DH
JANSSON, PE
LINDBERG, AA
机构
[1] HUDDINGE UNIV HOSP, KAROLINSKA INST, NOVUM, CLIN RES CTR, S-14186 HUDDINGE, SWEDEN
[2] KAROLINSKA INST, DEPT IMMUNOL MICROBIOL PATHOL & INFECT DIS, DIV CLIN BACTERIOL, S-14186 HUDDINGE, SWEDEN
[3] FISHERIES & OCEANS CANADA, SCI BRANCH, ST JOHNS, NF A1C 5X1, CANADA
关键词
D O I
10.1128/IAI.63.9.3683-3692.1995
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A murine monoclonal antibody, MAHI 3 (immunoglobulin G2b), that is broadly reactive with Haemophilus influenzae lipopolysaccharides (LPSs) but nonreactive with all enterobacterial LPSs tested was generated by fusing mouse myeloma cells with spleen cells of BALB/c mice immunized with azide-killed H. influenzae RM.7004. MAHI 3 bound to ail H. influenzae, all other human Haemophilus spp., all Bordetella pertussis and Bordetella parapertussis, and all Aeromonas spp. tested but not to any Neisseria or Moraxella catarrhalis strains, as determined by enzyme immunoassay, colony dot immunoblotting, and immunoblotting. In an inhibition enzyme immunoassay, MAHI 3 reacted with all 45 H. influenzae LPSs tested but not with the LPS from the rough mutant 169 Rd(-)/b(+), which has only 3-deoxy-D-manno-octulosonic acid (P) [Kdo(P)] and lipid A. The antibody was not inhibited by H. influenzae lipid A or lipid-free polysaccharide isolated after mild acid hydrolysis. Only native LPSs show positive inhibitory activity, indicating that]part of lipid A is involved in the binding of MAHI 3. From the results, it is indicated that the structural element recognized by MAHI 3 is Hep alpha 1-->2Hep alpha 1-->3Hep alpha 1-->Kdo together with part of lipid A, including the phosphate.
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页码:3683 / 3692
页数:10
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