DETECTION AND SUBCELLULAR-LOCALIZATION OF RABBIT PLATELET PHOSPHOLIPASE-A2 WHICH PREFERENTIALLY HYDROLYZES AN ARACHIDONOYL RESIDUE

被引：37

作者：

KIM, DK ^{[1
]}

KUDO, I ^{[1
]}

FUJIMORI, Y ^{[1
]}

MIZUSHIMA, H ^{[1
]}

MASUDA, M ^{[1
]}

KIKUCHI, R ^{[1
]}

IKIZAWA, K ^{[1
]}

INOUE, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,TOKYO 113,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 108卷 / 06期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123311

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Like rat platelets, rabbit platelets contain a secretory 14-kDa group II phospholipase A2 [Mizushima, H., Kudo, I., Horigome, K., Murakami, M., Hayakawa, M., Kim, D.K., Kondo, E., Tomita, M., & Inoue, K. (1989) J. Biochem. 105, 520-525]. The present study was undertaken to determine whether or not, in addition to that of the 14-kDa group II enzyme, rabbit platelets exhibit another phospholipase A2 activity. A rabbit platelet soluble fraction was prepared by sonication and centrifugation. When this soluble fraction was subjected to heparin-Sepharose column chromatography, phospholipase A2 activity was detected in both heparin-binding and heparin-non-binding fractions. The activity detected in the heparin-binding fraction appeared to belong to the secretory 14-kDa phospholipase A2, because it bound to anti-human 14-kDa group II phospholipase A2 monoclonal antibody. The activity found in the heparin-non-binding fraction did not appreciably react with the same antibody. When platelets were gently disrupted by the nitrogen cavitation method, the heparin-non-binding activity was mainly recovered in the platelet cytosolic fraction. The heparin-non-binding phospholipase A2 hydrolyzed a phospholipid bearing an arachidonoyl residue at the sn-2 position more effectively than one with a linoleoyl residue. The biochemical features of the activity observed in the heparin-non-binding fraction generally resembled those of human platelet soluble phospholipase A2 [Kim, D.K., Kudo, I., & Inoue, K. (1988) J. Biochem. 104, 492-494]. It can be concluded that rabbit platelets contain two kinds of phospholipase A2; one is the secretory 14-kDa group II phospholipase A2 in the granule fraction and the other is a cytosolic phospholipase A2 with a preference for arachidonoyl residues. © 1990 Copyright, 1990 by the Journal of Biochemistry.

引用

页码：903 / 906

页数：4

共 21 条

[1]

AARSMAN AJ, 1989, J BIOL CHEM, V264, P10008

[2] A CYTOSOLIC PHOSPHOLIPASE IN HUMAN-NEUTROPHILS THAT HYDROLYZES ARACHIDONOYL-CONTAINING PHOSPHATIDYLCHOLINE [J].