EXAMINATION OF THE BUOYANT DENSITY OF HEPATITIS-C VIRUS BY THE POLYMERASE CHAIN-REACTION

被引：23

作者：

CARRICK, RJ

SCHLAUDER, GG

PETERSON, DA

MUSHAHWAR, IK

机构：

[1] Experimental Biology Research, Abbott Laboratories, North Chicago, IL

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 39卷 / 03期

关键词：

HEPATITIS-C VIRUS; POLYMERASE CHAIN REACTION; HEPATITIS-C VIRAL RNA; BUOYANT DENSITY;

D O I：

10.1016/0166-0934(92)90101-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sucrose and cesium chloride density gradients were used to fractionate hepatitis C virus (HCV) infectious chimpanzee plasma. The fractionated plasma was then evaluated for HCV RNA sequences using cDNA synthesis and the polymerase chain reaction (cDNA/PCR). cDNA/PCR detectable HCV RNA was identified repeatedly in two regions. One region was at the top of the gradients with a buoyant density of less-than-or-equal-to 1.03 g/cm3, the other at a density of approximately 1.18-1.21 g/cm3.

引用

收藏

页码：279 / 290

页数：12

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