UPTAKE OF ORALLY-ADMINISTERED BETA-CAROTENE BY BLOOD-PLASMA, LEUKOCYTES, AND LIPOPROTEINS IN CALVES

被引:49
作者
CHEW, BP
WONG, TS
MICHAL, JJ
机构
[1] Department of Animal Sciences, Washington State University, Pullman
关键词
BETA-CAROTENE; LYMPHOCYTES; LIPOPROTEINS; BOVINE;
D O I
10.2527/1993.713730x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The uptake of beta-carotene by blood cells, plasma, and lipoproteins was studied in bull calves that were orally administered a single (Exp. 1; n = 18 Angus calves) or multiple (Exp. 2; n = 16 Holstein calves) doses of beta-carotene. Administration of beta-carotene increased plasma beta-carotene and the amount of beta-carotene associated with each lipoprotein fraction. Before beta-carotene treatment, the total amount of beta-carotene associated with the high-density lipoprotein (HDL) was three- to fourfold higher than the amount associated with low-density lipoprotein (LDL) and fivefold higher than the amount associated with very-low-density lipoprotein (VLDL). The relative increase in total beta-carotene associated with the lipoproteins was greater for LDL than for HDL or VLDL. Orally administered beta-carotene increased the uptake of beta-carotene by lymphocytes. Subcellular fractions of blood lymphocytes isolated from animals fed beta-carotene revealed that beta-carotene was taken up in significant amounts by the mitochondrial, nuclear, and microsomal fractions. The profile of beta-carotene uptake by these subcellular fractions did not mirror that observed in plasma. In contrast, beta-carotene was not detectable in blood neutrophils and erythrocytes in either beta-carotene-supplemented or unsupplemented calves. Treatment did not influence the concentrations of retinol or alpha-tocopherol in plasma, lipoproteins, lymphocytes, neutrophils, or erythrocytes. These data revealed the presence of beta-carotene in bovine lymphocyte subcellular fractions and suggest a possible physiological role of beta-carotene in these cells.
引用
收藏
页码:730 / 739
页数:10
相关论文
共 35 条
[1]   ORAL BETA-CAROTENE CAN INCREASE THE NUMBER OF OKT4+ CELLS IN HUMAN-BLOOD [J].
ALEXANDER, M ;
NEWMARK, H ;
MILLER, RG .
IMMUNOLOGY LETTERS, 1985, 9 (04) :221-224
[2]  
[Anonymous], 1991, PRINCIPLES PROCEDURE
[3]   CAROTENOIDS AND THE IMMUNE-RESPONSE [J].
BENDICH, A .
JOURNAL OF NUTRITION, 1989, 119 (01) :112-115
[4]   EFFECT OF BETA-CAROTENE AND CANTHAXANTHIN ON THE IMMUNE-RESPONSES OF THE RAT [J].
BENDICH, A ;
SHAPIRO, SS .
JOURNAL OF NUTRITION, 1986, 116 (11) :2254-2262
[5]  
BJORNSON LK, 1976, J LIPID RES, V17, P343
[6]  
BONDI A, 1984, PROG FOOD NUTR SCI, V8, P165
[7]   BETA-CAROTENE - AN UNUSUAL TYPE OF LIPID ANTIOXIDANT [J].
BURTON, GW ;
INGOLD, KU .
SCIENCE, 1984, 224 (4649) :569-573
[8]   KINETIC CHARACTERISTICS OF BETA-CAROTENE UPTAKE AFTER AN INJECTION OF BETA-CAROTENE IN PIGS [J].
CHEW, BP ;
WONG, TS ;
MICHAL, JJ ;
STANDAERT, FE ;
HEIRMAN, LR .
JOURNAL OF ANIMAL SCIENCE, 1991, 69 (12) :4883-4891
[9]   SUBCELLULAR-DISTRIBUTION OF BETA-CAROTENE, RETINOL, AND ALPHA-TOCOPHEROL IN PORCINE LYMPHOCYTES AFTER A SINGLE INJECTION OF BETA-CAROTENE [J].
CHEW, BP ;
WONG, TS ;
MICHAL, JJ ;
STANDAERT, FE ;
HEIRMAN, LR .
JOURNAL OF ANIMAL SCIENCE, 1991, 69 (12) :4892-4897
[10]   VITAMIN-A AND BETA-CAROTENE ON HOST DEFENSE [J].
CHEW, BP .
JOURNAL OF DAIRY SCIENCE, 1987, 70 (12) :2732-2743