PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE

被引：7

作者：

CURRAGH, HJ ^{[1
]}

MOOIBROEK, H ^{[1
]}

WESSELS, JGH ^{[1
]}

MARCHANT, R ^{[1
]}

MULLAN, E ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT PLANT BIOL,9751 NN HAREN,NETHERLANDS

来源：

MYCOLOGICAL RESEARCH | 1993年 / 97卷

关键词：

D O I：

10.1016/S0953-7562(09)81127-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.

引用

页码：313 / 317

页数：5

共 19 条

[1] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[2] TRANSFORMATION OF THE PHYTOPATHOGENIC FUNGUS SEPTORIA-NODORUM TO HYGROMYCIN-B RESISTANCE [J].

COOLEY, RN ;

SHAW, RK ;

FRANKLIN, FCH ;

CATEN, CE .

CURRENT GENETICS, 1988, 13 (05) :383-389

[3]

DWHALE SS, 1985, CURRENT GENETICS, V10, P205

[4] TRANSFORMATION IN FUNGI [J].

FINCHAM, JRS .

MICROBIOLOGICAL REVIEWS, 1989, 53 (01) :148-170

[5] PLASMID-ENCODED HYGROMYCIN-B RESISTANCE - THE SEQUENCE OF HYGROMYCIN-B PHOSPHOTRANSFERASE GENE AND ITS EXPRESSION IN ESCHERICHIA-COLI AND SACCHAROMYCES-CEREVISIAE [J].

GRITZ, L ;

DAVIES, J .

GENE, 1983, 25 (2-3) :179-188

[6] TRANSFORMATION OF FILAMENTOUS FUNGI [J].

HYNES, MJ .

EXPERIMENTAL MYCOLOGY, 1986, 10 (01) :1-8

[7] HYGROMYCIN-B RESISTANCE AS DOMINANT SELECTABLE MARKER IN YEAST [J].

KASTER, KR ;

BURGETT, SG ;

INGOLIA, TD .

CURRENT GENETICS, 1984, 8 (05) :353-358

[8] GENETIC-TRANSFORMATION OF THE FUNGAL PLANT WILT PATHOGEN, FUSARIUM-OXYSPORUM [J].

KISTLER, HC ;

BENNY, UK .

CURRENT GENETICS, 1988, 13 (02) :145-149

[9] ISOLATION AND REGENERATION OF PROTOPLASTS FROM FUSARIUM-TRICINCTUM AND FUSARIUM-OXYSPORUM [J].

LYNCH, PT ;

COLLIN, HA ;

ISAAC, S .

TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1985, 85 (AUG) :135-140

[10]

MANIATIS T, 1982, LABORATORY MANUAL

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