ALTERED PROCESSING CHARACTERISTICS OF BETA-AMYLOID-CONTAINING PEPTIDES IN CYTOSOL AND IN MEDIA OF FAMILIAL ALZHEIMERS-DISEASE CELLS

By pulse-chase analysis of cytosol and extracellular proteins, lymphoblastoid cells derived from patients with early- and late-onset familial Alzheimer's disease exhibit delayed and deficient processing characteristics of beta-amyloid precursor protein and its fragments. During 120 min chase incubation, 70-80% of the 16-kDa fragment with beta/A4-amyloid and cytoplasmic domains is removed in both cytosol and serum-free media of normal cells, whereas it remained unprocessed or even accumulated in familial Alzheimer's disease cells. Two-dimensional gel analysis further clarified that the 16-kDa peptide in familial Alzheimer's disease cells is highly phosphorylated compared with normals, and almost all of the accumulated 16-kDa preamyloid after 60 min chase incubation is phosphorylated in these cells. Tris-tricine gel analyis revealed a difference of processing characteristics between cytosol and extracellular beta/A4-containing peptides. Cytosol APP as the 16-kDa band in Tris-glycine gel was further separated into five peptides with molecular mass of 11 kDa-16.5 kDa, and some early-onset familial Alzheimer's disease cells contain 3-6 kDa peptides with beta/A4 domain, that are generated by proteolysis around transmembrane domain. Extracellular APP secreted during 24 h serum-free culture, however, exhibits only beta/A4-cytoplasmic domain-containing peptides with 12 kDa band as major component, but no 3-6 kDa peptides both in normal and familial Alzheimer's disease cells. These findings suggest that familial Alzheimer's disease lymphoblastoid cells harbour biochemical abnormality in the APP processing step to generate beta/A4-cytoplasmic domain peptides, and intracellular processing is crucial for eventual accumulation of beta/A4-amyloid in lymphoblastoid cells.