LP-805, A RELEASER OF ENDOTHELIUM-DERIVED NITRIC-OXIDE, ACTIVATES AN ENDOTHELIAL CALCIUM-PERMEABLE NONSPECIFIC CATION CHANNEL

被引：10

作者：

INAZU, M ^{[1
]}

ZHANG, H ^{[1
]}

DANIEL, EE ^{[1
]}

机构：

[1] MCMASTER UNIV, DEPT BIOMED SCI, DIV PHYSIOL & PHARMACOL, 1200 MAIN ST W, HAMILTON L8N 3Z5, ONTARIO, CANADA

来源：

LIFE SCIENCES | 1993年 / 53卷 / 19期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0024-3205(93)90626-E

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The actions of LP-805, a releaser of endothelium-derived nitric oxide, on Ca2+ permeable non-specific cation channel in cultured bovine pulmonary artery endothelial cells (BPAECs) were investigated using patch-clamp technique. In Ca2+ saline bath solution, 10 muM LP-805 increased inward and outward currents. LP-805 evoked currents were reversibly blocked by 50 muM La3+, an inorganic Ca2+ influx blocker. In Ca2+ and Na+ free saline bath solution, 10 muM LP-805 did not evoke these currents, when 2 mM Ca2+ was then added to the bath the inward current increased. Furthermore, in the Na+ saline bath solution (Ca2+ free), 10 muM LP-805 increased similar inward and outward currents, which were blocked by 50 muM La3+. LP-805 evoked currents were carried by either Ca2+ or Na+. With the pipette solution containing 11 mM EGTA, 10 muM LP-805 activated the inward and outward currents. The increase of inward and outward currents by 10 muM LP-805 was inhibited by 1 mM Ni2+ but not by 1 muM nicardipine or 50 muM SKF 96365. In conclusion, LP-805 increases Ca2+ influx into vascular endothelial cells through Ca2+ permeable non-specific cation channels. This may explain the release of endothelium-derived nitric oxide in endothelial cells by LP-805.

引用

页码：PL315 / PL320

页数：6

共 19 条

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