SEPARATION OF COMPLEX OLIGOSACCHARIDE MIXTURES BY CAPILLARY ELECTROPHORESIS IN THE OPEN-TUBULAR FORMAT

被引：90

作者：

STEFANSSON, M ^{[1
]}

NOVOTNY, M ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 07期

关键词：

D O I：

10.1021/ac00079a031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The fluorescent derivatives of complex oligosaccharide mixtures from different origins are separated in open capillaries. The number of negatively charged sulfonate groups in the tag molecule strongly affects separation efficiency and selectivity. Coating of the capillary surface with linear polyacrylamide has been essential to ensure fast and stable migration velocities. Efficiencies in excess of 1 million plates/m have been achieved, facilitating resolution of branched oligosaccharides. The effects of field strength and buffer composition on the apparent electrophoretic mobility of dextran and dextrin oligomers are discussed, with relation to borate complexation. Preliminary examples of applications to monitoring the action of hydrolytic and synthesizing enzymes are also described.

引用

页码：1134 / 1140

页数：7

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