COPPER-INHIBITED NADH-DEPENDENT PEROXIDASE-ACTIVITY OF PURIFIED SOYA BEAN PLASMA-MEMBRANES

被引：16

作者：

ZANCANI, M ^{[1
]}

NAGY, G ^{[1
]}

VIANELLO, A ^{[1
]}

MACRI, F ^{[1
]}

机构：

[1] UNIV UDINE,DIPARTIMENTO & ECON AGROIND,SEZ BIOL VEGETALE,I-33100 UDINE,ITALY

来源：

PHYTOCHEMISTRY | 1995年 / 40卷 / 02期

关键词：

GLYCINE MAX; LEGUMINOSAE; SOYA BEAN; PEROXIDASE; PLASMA MEMBRANE; COPPER;

D O I：

10.1016/0031-9422(95)00292-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly purified soya bean plasma membranes exhibited peroxidase activity. Divalent copper strongly inhibited NADH oxidation (but not H2O2 oxidation) catalysed by this enzyme, whereas Cd2+, Ni2+ or Zn2+ had little or no effect. This inhibition did not depend on a reaction by sulphydryl groups, or on a replacement of ions in the enzyme by CU2+. The effect of Cu2+ may be explained by its scavenging capability towards O-2(-) which is produced during NADH-dependent peroxidase activity.

引用

页码：367 / 371

页数：5

共 23 条

[1] NAD(P)H OXIDASE AND PEROXIDASE-ACTIVITIES IN PURIFIED PLASMA-MEMBRANES FROM CAULIFLOWER INFLORESCENCES [J].