ARABINOSE KINASE-DEFICIENT MUTANT OF ARABIDOPSIS-THALIANA

被引:31
作者
DOLEZAL, O [1 ]
COBBETT, CS [1 ]
机构
[1] UNIV MELBOURNE, DEPT GENET, PARKVILLE, VIC 3052, AUSTRALIA
关键词
D O I
10.1104/pp.96.4.1255
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A mutant of Arabidopsis thaliana that is sensitive to exogenous L-arabinose has been isolated. Comparisons of growth of the wild type, mutant, and F1 and F2 progeny of crosses showed the arabinose-sensitive phenotype is semidominant and segregates as a single Mendelian locus. Crosses of the mutant to marker strains showed the mutation is linked to the eceriferum-2 locus on chromosome 4. In vivo incorporation of exogenous labeled L-arabinose into ethanol-insoluble polysaccharides was greatly reduced in the mutant with a concomitant accumulation of free labeled arabinose. Enzyme assays of crude plant extracts demonstrated a defect in arabinose kinase activity in the mutant.
引用
收藏
页码:1255 / 1260
页数:6
相关论文
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